A case of insulin–likegrowthfactor2-producing gastrointestinal stromal tumor with severe hypoglycemia.
by Kayla
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Non-island cell tumor hypoglycemia (NICTH) is a rare paraneoplastic syndrome that secrete insulin molecule is not complete processing heavy high growth factor 2 (big-IGF2), which results in stimulation of insulin receptor and then inducing hypoglycemia. Gastrointestinal stromal tumor (GIST) is a common intestinal mesenchymal neoplasms of the gastrointestinal tract. Sites often the majority of GIST is the stomach; NICTH caused by stomach GISTs IGF2-producing is rare.An man 84 years was admitted to the hospital because of disturbance of consciousness (JCS II-10) in the morning. At the time of admission, his serum glucose was 44 mg / dL; consciousness was restored with 20 ml of 50% glucose. To avoid hypoglycemia, continuous intravenous infusion of glucose and dietary interventions are required.
At the time of hypoglycemia, insulin and C-peptide suppressed. In addition, IGF1 levels were below the normal range. Abdominal computed tomography revealed that he had a large lobulated mass (116 × 70 × 72 mm) around the corpus of the stomach.
pathological analysis of biopsy specimens messy identified spindle cells and positive for c-kit as well as a strong positive for the DOG-1. Further analysis revealed high levels of Ki-67 (Mib-1 Index: 15.5%) and mitotic index (7 / 50HPF); GIST tumors are diagnosed as high risk, and complete surgical resection performed.
Hypoglycemia be resolved after tumor resection. Resected tumor specimens positive for IGF2 staining, and large-IGF2 (11-18 kDa) was detected in the preoperative serum and tumor samples; patients diagnosed with NICTH for their IGF2-producing tumor.NICTH rare in GIST of the stomach; However, large GIST can produce large-IGF2 and subsequently cause severe hypoglycemia, requiring quick evaluation and complete tumor resection.
A case of insulin–likegrowthfactor2-producing gastrointestinal stromal tumor with severe hypoglycemia.
Downregulation of miR-637 promotes vascular smooth muscle cell proliferation and migration through regulation insulin – like growth factor – < em> 2 .
Dysregulation of proliferation and migration of vascular smooth muscle cells (VSMC) is an important cause of atherosclerosis. MiR-637 exerts antiproliferative effects on some human cells. impact on atherosclerosis remains largely unexplored.Real-time PCR was used to determine the expression of miR-637 in samples from patients and animal models of atherosclerosis. expression in VSMC dysfunction models (induced by ox-LDL) were measured. Proliferation and migration of VSMC were each tested by using CCK-8 and the test Transwell, and apoptosis was measured using flow cytometry.
The Targetscan database used to predict the miR-637 target genes. The interaction between miR-637 and a potential target genes validated by real-time PCR, Western blotting and luciferase reporter assay.MiR-637 significantly decreased the expression in atherosclerosis patients and animal model samples. It also decreased in a dose-and time-dependent in animal models of atherosclerosis ox-LDL-induced.
Description: A polyclonal antibody for detection of IGF2 from Human, Mouse, Rat. This IGF2 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human IGF2 protein
Description: A polyclonal antibody for detection of IGF2 from Human, Mouse, Rat. This IGF2 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human IGF2 protein
Description: A polyclonal antibody for detection of IGF2 from Human, Mouse, Rat. This IGF2 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human IGF2 protein
Description: This gene encodes a member of the insulin family of polypeptide growth factors| which are involved in development and growth. It is an imprinted gene| expressed only from the paternal allele| and epigenetic changes at this locus are associated with Wilms tumour| Beckwith-Wiedemann syndrome| rhabdomyosarcoma| and Silver-Russell syndrome. A read-through INS-IGF2 gene exists| whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene encodes a member of the insulin family of polypeptide growth factors| which are involved in development and growth. It is an imprinted gene| expressed only from the paternal allele| and epigenetic changes at this locus are associated with Wilms tumour| Beckwith-Wiedemann syndrome| rhabdomyosarcoma| and Silver-Russell syndrome. A read-through INS-IGF2 gene exists| whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene encodes a member of the insulin family of polypeptide growth factors| which are involved in development and growth. It is an imprinted gene| expressed only from the paternal allele| and epigenetic changes at this locus are associated with Wilms tumour| Beckwith-Wiedemann syndrome| rhabdomyosarcoma| and Silver-Russell syndrome. A read-through INS-IGF2 gene exists| whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Description: A polyclonal antibody for detection of IGF2-BP2 from Human. This IGF2-BP2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human IGF2-BP2 at AA rangle: 110-190
Description: A polyclonal antibody for detection of IGF2-BP2 from Human. This IGF2-BP2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human IGF2-BP2 at AA rangle: 110-190
Description: A polyclonal antibody for detection of IGF2-BP2 from Human. This IGF2-BP2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human IGF2-BP2 at AA rangle: 110-190
Description: IGF2BP2 encodes a member of the IGF-II mRNA-binding protein (IMP) family. The insulin like growth factor 2 mRNA binding protein 2 encoded by this gene contains four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternative promoter usage and alternate splicing result in multiple variants encoding different isoforms.
Description: IGF2BP2 encodes a member of the IGF-II mRNA-binding protein (IMP) family. The insulin like growth factor 2 mRNA binding protein 2 encoded by this gene contains four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternative promoter usage and alternate splicing result in multiple variants encoding different isoforms.
Description: IGF2BP2 encodes a member of the IGF-II mRNA-binding protein (IMP) family. The insulin like growth factor 2 mRNA binding protein 2 encoded by this gene contains four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternative promoter usage and alternate splicing result in multiple variants encoding different isoforms.
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human IGF2 (Center R54). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human IGF2 (aa81-93) (internal region). This antibody is tested and proven to work in the following applications:
Insulin Like Growth Factor 2 (IGF2) Polyclonal Antibody (Rat)
Transfection with miR-637 mimic suppressed proliferation and migration of VSMC while promoting apoptosis, whereas transfection with miR-637 inhibitor has the opposite effect. We also validated that the insulin-like growth factor-2 (IGF-2), an important factor in the pathogenesis of atherosclerosis, function as gene targets for miR-637.MiR-637 targets the IGF-2 contributes to the inhibition of atherosclerosis and could be a potential target for this illnesses.
