Fear extinction is an important preclinical model for behavioral therapy on human anxiety disorders, such as post-traumatic stress disorder (PTSD). Histone acetylation are involved in the extinction of fear memory. As a “reader” histone acetylation markers, role and domain bromodomain extraterminal (BET) protein in fear extinction remains unclear. In this study, we find that the emphasis BET proteins using small molecules JQ-1 has no effect on the acquisition of auditory fear or the fear of extinction recent hearing, but hearing the fear extinction interference distance.

We found that insulin-like growth factor 2 (IGF-2) mRNA and protein were up-regulated in the anterior cingulate cortex (ACC) after the fear memory extinction remote training, and that this effect is inhibited by JQ-1 administration. Furthermore, local delivery of the IGF-2 protein to save the ACC region remote memory extinction disruption caused by JQ-1 administration, which indicates IGF-2 mediates the effect on extinction JQ-1 remote memory.

Gene expression analysis showed that the JQ-1 treatment inhibits up-regulated expression of a key set of genes related to neuroplasticity following remote memory extinction profiles. Together, these findings establish BET as a mediator of epigenetic proteins for remote fear memory extinction. In particular, the findings of this study suggest that a preclinical cancer drug candidate, JQ-1 (or more BET bromodomain inhibitors) should be amended to prevent it from crossing the blood brain barrier and cause neurological side effects.

BET proteins inhibitor JQ-1 impaired the extinction of remote auditory fear memory: An effect mediated by <em>insulin</em> <em>like</em> <em>growth</em> <em>factor</em> <em>2</em>
BET proteins inhibitor JQ-1 impaired the extinction of remote auditory fear memory: An effect mediated by insulin like growth factor 2

Insulin – As Growth Factor two mRNA-Binding Protein 3 modulate the aggressiveness of Ewing sarcomas by CD164-CXCR4 Axis Set Ewing sarcoma (EWS) is the second most common bone and soft-tissue malignancy associated in children and young adults.

It is driven by the fusion oncogene EWS / FLI1 and is characterized by rapid growth and early metastasis. We have previously found that the mRNA binding protein IGF2BP3 is an important biomarker for EWS as IGF2BP3 high expression in primary tumors predicts poor prognosis of patients with EWS. We also show that IGF2BP3 improve anchorage-independent growth and cell migration EWS indicates that IGF2BP3 may work as a driver and molecular predictors of the development of EWS.

The purpose of this study was to further define IGF2BP3 role in the development of EWS. We show that high levels of mRNA expression correlated with EWS IGF2BP3 metastasis and disease progression in well-characterized tumor specimens EWS. EWS tumors with high levels of IGF2BP3 marked with the signature of a particular gene is enriched in chemokine-mediated signaling pathways. We also found that IGF2BP3 regulated CXCR4 expression via CD164. Significantly, CD164 and CXCR4 colocalized at the cell plasma membrane EWS on CXCL12 stimulation.

IGF2R antibody

70R-21570 50 ul
EUR 435
Description: Rabbit polyclonal IGF2R antibody

IGF2R Antibody

37644-100ul 100ul
EUR 252

IGF2R Antibody

1-CSB-PA005283
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against IGF2R. Recognizes IGF2R from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

IGF2R Antibody

1-CSB-PA632749
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against IGF2R. Recognizes IGF2R from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:50-1:200

IGF2R Antibody

DF8381 200ul
EUR 304
Description: IGF2R Antibody detects endogenous levels of total IGF2R.

IGF2R Antibody

1-CSB-PA016408
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against IGF2R. Recognizes IGF2R from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200

IGF2R antibody

70R-35061 100 ug
EUR 327
Description: Purified Rabbit polyclonal IGF2R antibody

IGF2R antibody

70R-36331 100 ug
EUR 327
Description: Rabbit polyclonal IGF2R antibody

IGF2R Antibody

1-CSB-PA011093GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
  • Form: Liquid
  • Buffer: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20℃, Avoid freeze / thaw cycles. Antigen Affinity purified
Description: A polyclonal antibody against IGF2R. Recognizes IGF2R from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB

IGF2R Antibody

1-CSB-PA011093LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against IGF2R. Recognizes IGF2R from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:20-1:200

IGF2R Antibody

1-CSB-PA009494
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against IGF2R. Recognizes IGF2R from Human, Mouse. This antibody is Unconjugated. Tested in the following application: IHC, IF, ELISA;IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/5000

IGF2R Antibody

1-CSB-PA009497
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against IGF2R. Recognizes IGF2R from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, IF, ELISA;IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/20000

IGF2R siRNA

20-abx920301
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

IGF2R siRNA

20-abx920302
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

IGF2R Antibody

ABD8381 100 ug
EUR 438

IGF2R/M6PR Antibody

48230-100ul 100ul
EUR 333

IGF2R/M6PR Antibody

48230-50ul 50ul
EUR 239

IGF2R Rabbit pAb

A13022-100ul 100 ul
EUR 308

IGF2R Rabbit pAb

A13022-200ul 200 ul
EUR 459

IGF2R Rabbit pAb

A13022-20ul 20 ul
EUR 183

IGF2R Rabbit pAb

A13022-50ul 50 ul
EUR 223

IGF2R Blocking Peptide

DF8381-BP 1mg
EUR 195

IGF2R antibody (Ser2409)

70R-35437 100 ug
EUR 327
Description: Purified Rabbit polyclonal IGF2R antibody (Ser2409)

IGF2R (pS2484) Antibody

abx216169-100ug 100 ug
EUR 439
  • Shipped within 5-10 working days.

IGF2R Conjugated Antibody

C37644 100ul
EUR 397

IGF2R-Specific Antibody

abx234174-100ug 100 ug
EUR 551
  • Shipped within 5-12 working days.

IGF2R (pS2409) Antibody

abx332987-100ul 100 ul
EUR 467
  • Shipped within 5-10 working days.

IGF2R Rabbit pAb

A19445-100ul 100 ul Ask for price

IGF2R Rabbit pAb

A19445-200ul 200 ul Ask for price

IGF2R Rabbit pAb

A19445-20ul 20 ul Ask for price

IGF2R Rabbit pAb

A19445-50ul 50 ul
EUR 308

Anti-IGF2R antibody

STJ11100639 50 µl
EUR 287
Description: This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611).

Anti-IGF2R antibody

STJ114989 100 µl
EUR 277
Description: This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611).

IGF2R (Phospho-Ser2409) Antibody

11708-100ul 100ul
EUR 252

IGF2R (Phospho-Ser2409) Antibody

11708-50ul 50ul
EUR 187

IGF2R (Phospho-Ser2484) Antibody

12443-100ul 100ul
EUR 252

IGF2R (Phospho-Ser2484) Antibody

12443-50ul 50ul
EUR 187

Human IGF2R ELISA kit

55R-2193 96 tests
EUR 766
Description: ELISA Kit for detection of IGF2R in the research laboratory

Phospho-IGF2R (Ser2409) Antibody

CSB-PA973142-
EUR 335
  • Form: liquid
  • Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodie
  • Show more
Description: A polyclonal antibody against Phospho-IGF2R (Ser2409). Recognizes Phospho-IGF2R (Ser2409) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:100

Phospho-IGF2R (Ser2409) Antibody

CSB-PA973142-100ul 100ul
EUR 362
  • Form: liquid
  • Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodie
  • Show more
Description: A polyclonal antibody against Phospho-IGF2R (Ser2409). Recognizes Phospho-IGF2R (Ser2409) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:100

IGF2R (Phospho-Ser2409) Antibody

20-abx012519
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • Shipped within 5-10 working days.

IGF2R ELISA KIT|Human

EF006931 96 Tests
EUR 689

IGF2R/M6PR Conjugated Antibody

C48230 100ul
EUR 397

IGF2R Antibody, HRP conjugated

1-CSB-PA011093LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against IGF2R. Recognizes IGF2R from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

IGF2R Antibody, FITC conjugated

1-CSB-PA011093LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against IGF2R. Recognizes IGF2R from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

IGF2R Antibody, Biotin conjugated

1-CSB-PA011093LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against IGF2R. Recognizes IGF2R from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Phospho-IGF2R (S2409) Antibody

1-CSB-PA009491
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against Phospho-IGF2R (S2409). Recognizes Phospho-IGF2R (S2409) from Human, Mouse, Monkey. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/10000

Phospho-IGF2R (Ser2484) Antibody

AF8068 200ul
EUR 376
Description: IGF2R (Phospho-Ser2484) Antibody detects endogenous levels of IGF2R only when phosphorylated at Ser2484.

Human IGF2R shRNA Plasmid

20-abx952353
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Mouse IGF2R shRNA Plasmid

20-abx970988
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

IGF2R (Phospho- Ser2484) Antibody

ABF8068 100 ug
EUR 438

anti- IGF2R-Specific antibody

FNab04174 100µg
EUR 585
  • Recommended dilution: WB: 1:1000-1:4000
  • IP: 1:500-1:1000
  • IHC: 1:20-1:200
  • IF: 1:20-1:200
  • Immunogen: insulin-like growth factor 2 receptor
  • Uniprot ID: P11717
  • Research Area: Cancer, Immunology, Signal Transduction
Description: Antibody raised against IGF2R-Specific

Anti-IGF2R-Specific antibody

PAab04174 100 ug
EUR 412

ELISA kit for Human IGF2R

EK5606 96 tests
EUR 553
Description: Enzyme-linked immunosorbent assay kit for quantification of Human IGF2R in samples from serum, plasma, tissue homogenates and other biological fluids.

Human IGF2R PicoKine ELISA Kit

EK1326 96 wells
EUR 455
Description: For quantitative detection of human IGF2R in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Phospho-IGF2R (Ser2484) Blocking Peptide

AF8068-BP 1mg
EUR 195

Igf2r ORF Vector (Rat) (pORF)

ORF068545 1.0 ug DNA
EUR 2693

IGF2R ORF Vector (Human) (pORF)

ORF021398 1.0 ug DNA Ask for price

Igf2r ORF Vector (Mouse) (pORF)

ORF047735 1.0 ug DNA
EUR 2697

Anti-IGF2R (Phospho-Ser2484) Antibody

STJ193219 200 µl
EUR 197

IGF2R ELISA Kit (Human) (OKBB00663)

OKBB00663 96 Wells
EUR 544
Description: Description of target: Insulin-like growth factor 2 receptor, also called IGF2R or I-MPR is a protein that in humans is encoded by the IGF2R gene. This gene is mapped to 6q25.3. This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: <= 10 pg/mL

IGF2R ELISA Kit (Human) (OKCD07535)

OKCD07535 96 Wells
EUR 936
Description: Description of target: This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611).;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.221ng/mL

IGF2R ELISA Kit (Bovine) (OKEH08399)

OKEH08399 96 Wells
EUR 1092
Description: Description of target: ;Species reactivity: Bovine;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.089ng/mL

IGF2R ELISA Kit (Human) (OKEH04584)

OKEH04584 96 Wells
EUR 662
Description: Description of target: This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611).;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.174 ng/mL

IGF2R ELISA Kit (Mouse) (OKEH04275)

OKEH04275 96 Wells
EUR 662
Description: Description of target: Acts as a positive regulator of T-cell coactivation, by binding DPP4 (By similarity). Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.087 ng/mL

Polyclonal IGF2R / CD222 Antibody (aa2441-2490)

APR16806G 0.05ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human IGF2R / CD222 (aa2441-2490). This antibody is tested and proven to work in the following applications:

IGF2R (Phospho-Ser2409) Polyclonal Conjugated Antibody

C11708 100ul
EUR 397

IGF2R (Phospho-Ser2484) Polyclonal Conjugated Antibody

C12443 100ul
EUR 397

Igf2r sgRNA CRISPR Lentivector set (Rat)

K7527601 3 x 1.0 ug
EUR 339

Igf2r sgRNA CRISPR Lentivector set (Mouse)

K4677201 3 x 1.0 ug
EUR 339

IGF2R sgRNA CRISPR Lentivector set (Human)

K1025101 3 x 1.0 ug
EUR 339

Anti-Mannose 6 Phosphate Receptor/Igf2r Antibody

A00951-1 100ug/vial
EUR 334

Igf2r sgRNA CRISPR Lentivector (Rat) (Target 1)

K7527602 1.0 ug DNA
EUR 154

Igf2r sgRNA CRISPR Lentivector (Rat) (Target 2)

K7527603 1.0 ug DNA
EUR 154

Igf2r sgRNA CRISPR Lentivector (Rat) (Target 3)

K7527604 1.0 ug DNA
EUR 154

Igf2r sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4677202 1.0 ug DNA
EUR 154

Igf2r sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4677203 1.0 ug DNA
EUR 154

Igf2r sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4677204 1.0 ug DNA
EUR 154

IGF2R sgRNA CRISPR Lentivector (Human) (Target 1)

K1025102 1.0 ug DNA
EUR 154

IGF2R sgRNA CRISPR Lentivector (Human) (Target 2)

K1025103 1.0 ug DNA
EUR 154

IGF2R sgRNA CRISPR Lentivector (Human) (Target 3)

K1025104 1.0 ug DNA
EUR 154

IGF2R Protein Vector (Human) (pPB-C-His)

PV085589 500 ng Ask for price

IGF2R Protein Vector (Human) (pPB-N-His)

PV085590 500 ng Ask for price

IGF2R Protein Vector (Human) (pPM-C-HA)

PV085591 500 ng Ask for price

IGF2R Protein Vector (Human) (pPM-C-His)

PV085592 500 ng Ask for price

IGF2R Protein Vector (Rat) (pPB-C-His)

PV274178 500 ng
EUR 4028

IGF2R Protein Vector (Rat) (pPB-N-His)

PV274179 500 ng
EUR 4028

IGF2R Protein Vector (Rat) (pPM-C-HA)

PV274180 500 ng
EUR 4028

IGF2R Protein Vector (Rat) (pPM-C-His)

PV274181 500 ng
EUR 4028

IGF2R Protein Vector (Mouse) (pPB-C-His)

PV190938 500 ng
EUR 4034

IGF2R Protein Vector (Mouse) (pPB-N-His)

PV190939 500 ng
EUR 4034

IGF2R Protein Vector (Mouse) (pPM-C-HA)

PV190940 500 ng
EUR 4034

IGF2R Protein Vector (Mouse) (pPM-C-His)

PV190941 500 ng
EUR 4034

Igf2r 3'UTR Luciferase Stable Cell Line

TU109963 1.0 ml Ask for price

We further showed that the expression level IGF2BP3, CD164, and CXCR4 correlated in clinical samples and IGF2BP3 that / CD164 / CXCR4 signaling pathway promoted EWS cell motility in response to CXCL12 and under hypoxic conditions. Data presented are identified CD164 and CXCR4 as a novel functional IGF2BP3 downstream effectors showed that IGF2BP3 / CD164 / CXCR4 axis oncogenic may work as critical modulators EWS aggressiveness. In addition, expression levels IGF2BP3, CD164, and CXCR4 could be a novel predictive biomarker panel EWS development.