Effect of Dexmedetomidine-Mediated Insulin–LikeGrowthFactor2 (IGF2) Signal Pathway on Immune Function and Invasion and Migration of Cancer Cells in Rats with Ovarian Cancer.
by Kayla
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BACKGROUND The purpose of this study was to explore the effects of dexmedetomidine (DEX) -mediated insulin-like growth factor 2 (IGF2) signaling pathway in immune functions and cancer cell invasion and migration in mice with ovarian cancer. MATERIALS AND METHODS Forty mice with ovarian cancer were divided into 4 groups: the model group and low dose (0.2 mg / kg / h DEX), medium dose (1.0 ug / kg / h DEX), and high dose ( 5.0 mg / kg / h DEX) DEX group. In addition, 10 Fischer344 rats selected as normal group.
Human NUTU 19 poorly differentiated epithelial ovarian cancer cells were divided into 4 groups: empty and low-dose, medium-dose and high-dose DEX NUTU-19 group. RESULTS Compared with normal group, other groups serum interleukin (IL) -2 and interferon gamma (INF-γ) levels, CD4⁺ and CD8⁺ percentages, ratios CD4⁺ / CD8⁺, and spleen lymphocyte transformation rate decreases, and the alpha serum tumor necrosis factor (TNF-alpha) levels, IGF2, insulin-like growth factor 1 receptor (IGF1R), insulin receptor substrate 1 (IRS1) mRNA and protein expression in ovarian tissue increased (all P <0.05).
Results in the DEX group compared with the model group are the opposite of those in the control group compared with the normal group (all P <0.05). Compared with blank group, other groups proliferation, invasion, and migration of ovarian cancer cells reduced significantly (all P <0.05). Compared with low doses of DEX NUTU-19 groups, in high doses of DEX-19 group NUTU invasion and migration of ovarian cancer cells weakened significantly (both P <0.05).
Conclusion A certain dose DEX can effectively inhibit the activation of signaling pathways IGF2 to enhance the immune function of mice with ovarian cancer, inhibits invasion and migration of ovarian cancer cells.
Effect of Dexmedetomidine-Mediated Insulin–LikeGrowthFactor2 (IGF2) Signal Pathway on Immune Function and Invasion and Migration of Cancer Cells in Rats with Ovarian Cancer.
Insulin-like growth factor II mRNA binding protein 3 promotes cell proliferation, migration and invasion in human glioblastoma.
Background / Purpose: Recently, insulin-like growth factor-binding protein mRNA 3 (IMP3) has been reported to be involved in tumorigenesis. We aim to study the expression and role of IMP3 in human glioblastoma. Methods: We analyzed the expression of IMP3 in 70 cases of glioma tissue, normal brain tissue and 5 of cell lines using western blot. Immunohistochemistry (IHC) was used to evaluate the expression and distribution of IMP3 in glioma tissue. colony formation, wound healing, migration and invasion tests and tumorigenesis in nude mice were used to explore the IMP3 function in vitro and in vivo.
The epithelial-mesenchymal transition (EMT) -related biomarkers detected by Western blot. Results: We found that the expression levels of IMP3 was significantly higher in glioma tissue than that in normal brain tissue and glioma associated with the class. In-vitro tests revealed that excessive IMP3 was significantly induced cell proliferation, migration, and invasion. Mechanically, IMP3 over-expression downregulated the expression of E-cadherin, but upregulated the expression of N-cadherin, vimentin, snails, slugs and MMP9.
However, inhibition of oncogenic effects IMP3 disorders. In vivo assay also showed that silencing of IMP3 inhibit tumor growth and increased survival of tumor-bearing nude mice xenograft. Conclusion: IMP3 can promote cell proliferation, migration and invasion by inducing EMT in glioblastoma. Thus, targeting the pathway IMP3 may be a new way to treat patients with glioblastoma.Schizophrenia associated with abnormal neural development of the brain, the IGF-2 (insulin-like growth factor-2) had a major impact.
Description: The IGF1 receptor binds insulin-like growth factor with a high affinity and plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. The protein possess tyrosine kinase activity.
Description: IGF1R is a tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1 receptor is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis.
Description: IGF1R (Insulin-like Growth Factor 1 (IGF-1) Receptor) is a protein found on the surface of human cells. It is a transmembrane receptor that is activated by a hormone called Insulin-like growth factor 1 (IGF-1) and by a related hormone called IGF-2. It belongs to the large class of tyrosine kinase receptors. The IGF1R gene is mapped on 15q26.3. IGF-1 plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues. Using a yeast 2-hybrid system, it was identified a regulatory subunit of phosphatidylinositol (PI) 3-kinase, PIK3R3, as a binding partner of IGF1R. Functional interaction between BRCA1 and SP1 in the regulation of the IGF1R gene was studied in Schneider cells, a Drosophila cell line which lacks endogenous SP1. In these cells, BRCA1 suppressed 45% of the SP1-induced trans-activation of the IGF1R promoter. Overexpression of the Grb10-binding fragment of Gigyf1 resulted in a significant increase in Igf1-stimulated Igf1r tyrosine phosphorylation. Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning it signals by causing the addition of a phosphate molecule on particular tyrosines. IGF-1 activates the Insulin receptor at approximately 0.1x the potency of insulin. Part of this signaling may be via IGF1R-InsulinReceptor heterodimers.
Description: A Monoclonal antibody against Human IGF1R / IGF1 Receptor. The antibodies are raised in Mouse. This antibody is applicable in WB and IHC-P, E, Flo
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human IGF1R / IGF1 Receptor (aa1126-1175). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human IGF1 Receptor (IGF1R) (N-term). This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human IGF1 Receptor (IGF1R) (C-term). This antibody is tested and proven to work in the following applications:
Description: A Monoclonal antibody against Human IGF1R / IGF1 Receptor (clone 1H7). The antibodies are raised in Mouse and are from clone 1H7. This antibody is applicable in WB and IHC-P, Flo
Description: A Monoclonal antibody against Human IGF1R / IGF1 Receptor (aa1264-1367, clone JBW902). The antibodies are raised in Mouse and are from clone JBW902. This antibody is applicable in WB and IHC-P, IP
Description: A polyclonal antibody against IGF1. Recognizes IGF1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/10000
Description: A polyclonal antibody against IGF1. Recognizes IGF1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200
Description: A polyclonal antibody against IGF1. Recognizes IGF1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200
Description: A polyclonal antibody against IGF1. Recognizes IGF1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:200-1:500
Description: A polyclonal antibody against IGF1. Recognizes IGF1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100
Description: The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.
Description: The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.
Description: Glucocorticoid receptor is a protein encoded by the NR3C1 gene which is approximately 85,6 kDa. The glucocorticoid receptor is localised to the cytoplasm, mitochondrion and nucleus. It is involved in PEDF induced signalling, gene expression and the nuclear receptor transcription pathway. It is a protein which can function both as a transcription factor that binds to glucocorticoid response elements to activate their transcription, and as a regulator of other transcription factors. The glucocorticoid receptor is widely expressed. Mutations in the NR3C1 gene may result in glucocorticoid resistance. STJ97101 was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. This antibody detects endogenous GR proteins.
Description: Rabbit Polyclonal Cannabinoid Receptor 1 Antibody. Validated in IF, IHC and tested in Human, Mouse, Rat.
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The purpose of this study was to evaluate serum levels and IGF-2 binding protein IGFBP-3 and IGFBP-7 in patients with schizophrenia and the association of this protein with schizophrenia psychopathology and cognitive deficits
